Cryosectioning Tissues

Cryosectioning tissues.

INTRODUCTIONCryosections are rapidly and relatively easily prepared prior to fixation, and they provide a good system for visualizing fine details of the cell. Although cryosections are physically less stable than paraffin- or resin-embedded sections, they are generally superior for the preservation of antigenicity and therefore the detection of antigens by microscopy. The preparation of cryose...

Flash freezing and cryosectioning E12.5 mouse brain.

Cryosectioning yeast communities for examining fluorescence patterns.

Microbes typically live in communities. The spatial organization of cells within a community is believed to impact the survival and function of the community(1). Optical sectioning techniques, including confocal and two-photon microscopy, have proven useful for observing spatial organization of bacterial and archaeal communities(2,3). A combination of confocal imaging and physical sectioning of...

Cryosectioning Method for Microdissection of Murine Colonic Mucosa

The colonic mucosal tissue provides a vital barrier to luminal antigens. This barrier is composed of a monolayer of simple columnar epithelial cells. The colonic epithelium is dynamically turned over and epithelial cells are generated in the stem cell containing crypts of Lieberkühn. Progenitor cells produced in the crypt-bases migrate toward the luminal surface, undergoing a process of cellula...

A new and simple cryosectioning protocol for pollen analysis under light microscopy

Conventional plant microtechniques typically involve fixation, dehydration, and embedding in acrylic or epoxy resins followed by room temperature microtomy. Some cryomethods have been used for pollen grain analysis under light microscopy. However, these protocols do not allow for obtaining sections with similar or better quality compared to resin embedding methods. In this paper, a simple cryop...